Conservation of somatic muscle produced from collared peccaries (Pecari tajacu Linnaeus, 1758) making use of direct or vitrification that is solid-surface

Conservation of somatic muscle produced from collared peccaries (Pecari tajacu Linnaeus, 1758) making use of direct or vitrification that is solid-surface

Affiliations

  • 1 Laboratory of Animal Biotechnology, Federal Rural University of Semiarid, Mossoro, 59625900, Brazil.
  • 2 Laboratory of Animal Germplasm Conservation, Federal Rural University of Semiarid, Mossoro, 59625900, Brazil.
  • 3 Laboratory of Applied Animal Morphophysiology, Federal Rural University of Semiarid, Mossoro, 59625900, Brazil.
  • 4 Laboratory of Animal Biotechnology, Federal Rural University of Semiarid, Mossoro, 59625900, Brazil. [email protected].
  • PMID: 28260212
  • PMCID: PMC5507844
  • DOI: 10.1007/s10616-017-0074-7

Free PMC article

Conservation of somatic muscle based on collared peccaries (Pecari tajacu Linnaeus, 1758) utilizing direct or vitrification that is solid-surface

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Writers

Affiliations

  • 1 Laboratory of Animal Biotechnology, Federal Rural University of Semiarid, Mossoro, 59625900, Brazil.
  • 2 Laboratory of Animal Germplasm Conservation, Federal Rural University of Semiarid, Mossoro, 59625900, Brazil.
  • 3 Laboratory of Applied Animal Morphophysiology, Federal Rural University of Semiarid, Mossoro, 59625900, Brazil.
  • 4 Laboratory of Animal Biotechnology, Federal Rural University of Semiarid, Mossoro, 59625900, Brazil. [email protected].
  • PMID: 28260212
  • PMCID: PMC5507844
  • DOI: 10.1007/s10616-017-0074-7

Abstract

Cryopreservation of somatic muscle could be used in biodiversity preservation, specifically for crazy species as collared peccary. We aimed to judge the end result of vitrification strategies of ear muscle of collared peccary [direct vitrification in cryovials (DVC) or solid-surface vitrification (SSV)] in the levels of epidermis and dermis by main-stream histology and cellular cap cap ability through the inside vitro tradition. Therefore, both the vitrification techniques had the ability to keep normal habits associated with epidermis once the cornea and granular levels, moreover the intercellular room and dermal-epidermal junction associated with spinous layer compared to control that is fresh. However, DVC and SSV portion of normality reduced within the integrity that is morphological of (37.5 and 25.0%) of spinous layer, correspondingly, when compared with the new fragments (100%, p 0.05). In addition, just at time of 72 h (D3), within the development bend, DVC fragments revealed a cell that is reduced than fresh control. In closing, SSV ended up being discovered become an even more method that is efficient vitrifying collared peccary epidermis muscle compared to DVC. These email address details are appropriate for the muscle cryopreservation from collared peccary and may additionally be ideal for animals with phylogenetic relationships.

Keyword phrases: Cryopreservation; Dermis; Epidermis; In vitro tradition; Somatic cellular; wildlife.

Figures

Histological analysis of ear muscle…

Histological analysis of ear muscle of collared peccaries in fresh control ( a…

In vitro culture of ear…

In vitro tradition of ear muscle of collared demonstrating that is peccary, adhesion, and…

The rise bend of epidermis…

The rise bend of epidermis cells produced from somatic muscle of collared peccary…

Attractive People Attract More Attention…to Their Particular Personality Traits

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